Project

Part:BBa_K329022

Designed by: Raphael Pantier   Group: iGEM10_Paris_Liliane_Bettencourt   (2010-10-24)


Population counter expressing RFP - PmS-RFP

This part is one of our population counters. When IntI1 (BBa_K329014) is expressed, it excises the double terminator flanked by attC sites (BBa_J99001) and triggers the expression of mRFP1 because of the constitutive promoter upstream.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1125
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 1125
    Illegal NotI site found at 2001
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1125
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1125
    Illegal AgeI site found at 1704
    Illegal AgeI site found at 1816
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None